Human colon cancer-specific antigen-2, CCSA-2 ELISA Kit from MyBioSource.com

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Human colon cancer-specific antigen-2, CCSA-2 ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Colon Cancer-specific Antigen-2 (also known as CCSA-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CCSA-2 antibody. CCSA-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CCSA-2 Antibody is added and binds to CCSA-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCSA-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CCSA-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm